Up at 7am this morning as I must go to Wastewater treatment plant and get some sludge, mmm. Smells really bad, but it contains bacteria for my experiments. The bacteria converts nitrate (NO3) to nitrogen gas (N2). The process is not always so simple, some intermediates can result. These intermediates can cause problems when they accumulate, mmm, cause thats bad, mmmmmkay? :)